Tissue Engineering and Regenerative Medicine / Phuc Van Pham, editor.

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2.2.2 ECM and Tendon Regeneration

Academic

Intro; Contents; Contributors; Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro; 1 Introduction; 2 Materials and Methods; 2.1 Materials; 2.2 Pellet Fabrication; 2.3 Diametral Tensile Strength (DTS) Testing; 2.4 Density Testing; 2.5 Pellet Sterilization; 2.6 Evaluation of Cell Toxicity; 2.7 Cell Attachment Assay; 2.8 Field-Emission Scanning Electron Microscopy with Energy-Dispersive X-Ray Spectroscopy (FESEM-EDX) Analysis for Surface of ...; 2.9 Statistical Analysis; 3 Results; 3.1 Pellet Structure and Morphology

3.2 Cell Toxicity Assay3.3 Osteoblast Proliferation and Attachment Assay; 3.4 FESEM-EDX Analysis of the Pellets ́Surface; 4 Discussion; 5 Conclusion; References; The Robust Potential of Mesenchymal Stem Cell-Loaded Constructs for Hard Tissue Regeneration After Cancer Removal; 1 Introduction; 2 Various Strategies Employed in TE for Hard Tissue Replacement; 2.1 Cell-Free Scaffolds Strategy; 2.2 Bioactive-Loaded Scaffolds Strategy; 2.3 Cell-Based Scaffold Strategy; 3 Requirements of Hard Tissue-Engineered Scaffolds; 3.1 Biocompatibility; 3.2 Biodegradability

3.3 Porosity, Pore Size, and Level of Pore Interconnectivity3.4 Surface Chemistry; 3.5 Mechanical Properties; 3.6 Osteoimmunomodulation; 4 Effective Parameters in the Reciprocal Cell-Scaffold Interaction; 4.1 Cell-Mediated Remodeling of Scaffolds; 4.2 Influence of Scaffold on Cellular Behaviors; 5 Cell-Loading Approaches; 6 Cell-Loaded Constructs in Clinical and Preclinical Settings; 7 Concluding Remarks; References; In Vitro Production of Cartilage Tissue from Rabbit Bone Marrow-Derived Mesenchymal Stem Cells and Polycaprolactone Scaffold; 1 Introduction; 2 Materials-Methods

2.1 Fabrication of Biodegradable Scaffolds2.2 Isolation and Characterization of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells (BMMSCs); 2.3 Production of BMMSCs Expressing Green Fluorescent Protein (GFP-BMMSCs); 2.4 Seeding BMMSCs onto PCL Scaffolds; 2.5 Cell Proliferation Assay; 2.6 In Vitro Cartilage Formation; 2.7 Scanning Electron Microscopy (SEM); 2.8 Gene Expression Evaluation; 2.9 Statistical Analysis; 3 Results; 3.1 Polycaprolactone Scaffold Preparation; 3.2 Successful Isolation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells; 3.3 BMMSCs Were Successfully Transfected with GFP

3.4 BMMSCs Could Adhere and Proliferate on PCL Scaffolds and Collagen-Coated PCL Scaffolds3.5 BMMSCs Were Differentiated into Chondrocytes in the Scaffold; 4 Discussion; 5 Conclusion; References; Stem Cell Therapy for Tendon Regeneration: Current Status and Future Directions; 1 Introduction; 1.1 Tendon Injuries and Healing; 2 Application of Stem Cells, GFs, and Transcription Factors in Tendon Injury Therapies; 2.1 Stem Cells; 2.1.1 Other Important Cell Sources for Tendon Healing; 2.2 GFs in Tendon Development and Healing; 2.2.1 Effects of Different GFs on Tendon Healing

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